Wednesday, January 7, 2015

The principle of antisense technology top is the normal protein synthesis, forming the mRNA copy (=

Synthetic DNA
Synthetic mimics of DNA and RNA can be an important element in the development of modern drugs. Locked Nucleic Acid (LNA) is one such synthetic DNA molecule, as there are high hopes for. Read the original article here
Modern scientific research often move in the border area between established disciplines. This applies particularly to the development of synthetic imitations of nature's own nucleic acids DNA and RNA, which takes place on the border between chemistry and biology. There is great interest in this research - partly because it can give us a better understanding of chemical and biological processes at the molecular level, fssai but also because the synthetic DNA predicted a great future for the development of new drugs and diagnostics. The DNA molecule in the cell nucleus occurs primarily as a double helix consisting of two DNA strands. DNA's building blocks are four nucleosides, referred to as A, T, C and G ('the four bases') where, for example. A of one strand specifically binds to (recognizes =) T in the other. The same is true for C and G.
Drugs with no side effects fssai A problem with many drugs is that they not only works where the cause of the disease is available, so that side effects occur. An example is chemotherapy, which using. Chemical compound trying fssai to inhibit the proliferation of cancer cells. Unfortunately, the chemical compound inhibits not only the growth of cancer cells but also normal cells of the body. Therefore, there is a great need for developing more selective drugs that act specifically on the cause of the disease and only there. A large number of known disorders can be attributed to one or more disease-related proteins (eg. Receptors or enzymes), which is formed by the body. The protein may, for example. included as a necessary element for the building, and thus for the propagation of HIV virus particles, or it may lead to the development of cancer, as such. In certain circumstances, the so-called ras proteins which are involved in cell growth. Traditionally in the development of drugs work to interfere in the biochemical fssai processes that occur in the body's proteins. An example is the combination treatment of HIV infection that occurs with various substances that inhibit some of the enzymes that are important for the propagation of the HIV virus (respectively enzyme HIV reverse transcriptase and HIV protease).
Antisense drugs In particular, to avoid side effects were developed in the late eighties a new revolutionary fssai therapeutic principle: Antisense drugs. The principle is to slow the disease fssai at source fssai by preventing the production of harmful protein. In practice, it works by inhibiting the expression of the genes encoding the harmful proteins. In addition to design a synthetic DNA strand (antisense DNA) so it just binds (that makes the double-strand) to the messenger RNA that forms the template for the harmful fssai protein. In this way, the formation of the protein is blocked (Figure 1). Compared with the above example, with HIV infection will specifically be able to prevent the formation (i.e. biosynthesis) of the two enzymes, thereby preventing the multiplication of the HIV virus. fssai The first antisense drug was last approved in the US for the treatment of CMV infection (a viral infection of the eye, in particular, occur in AIDS patients).
The principle of antisense technology top is the normal protein synthesis, forming the mRNA copy (= messenger RNA) of a gene in the nuclear DNA. The mRNA copy transported out of the nucleus to the ribosomes in the cytoplasm. Here, the mRNA is read and translated into the amino acids that are joined together in a long chain, wherein a protein fssai is formed. It is this process which antisense DNA interferes by binding to mRNA before fssai it is translated in the ribosome. Thus blocking protein synthesis, and genetic diseases can be slowed.
The ideal characteristics of synthetic DNA It is not possible to use ordinary (ie chemically unmodified DNA or RNA strands) as antisense drugs, with natural enzymes (nucleases) in a short time will break down these for body foreign molecules. Therefore, over the past 10 years in research laboratories around the world tried to develop synthetic DNA and RNA strands with the right properties. Despite intensive research efforts, which have resulted in the synthesis of over 1000 chemically different synthetic DNA mimics, have failed to produce a string with the right properties. The ideal nucleic acid imitation must be able to bind strongly and with high precision (i.e., with correct base-base recognition) to its complementary sequence. Further, these binding properties combined with, for example. water solubility, ability to penetrate through the membranes and resistance to enzymatic degradation.
LNA - a good imitation We started our research in the field with the desire to develop a nucleic acid imitation, which would meet these requirements. Our approach t

No comments:

Post a Comment